conversion of nucleotide sequence to aminoacid sequence

Question

[ashamscsoft]

I have one nucleotide sequence. I need to change that sequence to amino acid sequence. To convert there are many tools, but how do we know that the sequence converted to amino acid sequence is correct or not. I'm getting different sequence when i try it in different tools. Could someone help in this.

[jotan]

What organism is the gene sequence from? Are there introns? What tools have you tried? What do the outputs look like?

[ashamscsoft]

The organism is homo sapiens. The sequence is cDNA sequence in that there wont be introns. I tried in expasy, EMBL-EBI and sequence manipulation suite and other normal conversions.

[jotan]

Seems pretty straightforward, it's hard to understand what might be going wrong. Could you show the sequence and/or conversions?

[Michael]

Hi ashamscsoft, if you need more specific help please add an example to your question of a sequence that is translated differently. There are few parameters that influence translation by tools like transeq, see also A: Translating DNA to protein with perl for reasons where implementation could differ.

However, if the genetic code is the same, the frame and strand are the same, and the input is the same, then the output of all tools should also be identical. If not maybe one or all are at fault.

[ashamscsoft]

thanks will check it

Answer 1

[archana.bioinfo87]

You can use emboss tool for DNA to protein conversion using http://web.expasy.org/translate/ Hope it will help you.

[ashamscsoft]

I have a doubt in that tool if we give our sequences, it gives the output as frames. how we wil get full amino acid sequences L C V I T A Q Q K K F H S D A Q K F L K F W R I S D R L C H Y V F T Y C Q like this