Visualize RSEM Results/Normalization

Question

[yrc0313]

So I ran RSEM and got some data. I just want to check and make sure that my FPKM values are normalized. So I used excel (bar graph) and plotted the log2(FPKM+1). What I got ended up being half of a bell curve, with all values equal or greater to 0. This makes sense in my mind but I would just like to confirm that this process is right or if there is a better way of confirming that the results are normalized correctly. Thank you!

[Alex Reynolds]

You might look at a QQ plot.

Answer 1

[Charles Warden]

What does log2(FPKM+0.1) look like? Including smaller values should round out your bell curve.