WGCNA module significance ?

Question

[Nitin]

Hello,

I Recently performed WGCNA analysis of microarray dataset. This dataset consists of two following conditions

1) Untreated 2) treated

WGCNA analysis revealed that there 10 co-expression modules. Now I am thinking to identify the significant module among these 10. I went through the tutorials there are two measures Gene significance and Module significance. But these measures are calculated by relating each module with trait file (like phenotypic features or weight and so on). I also want to do this but I donot have any external information to related to expect two condition information like Untreated and treated. Can any body give suggestion how to determine module significance with out external data?

Thanks a lot,

Sai

[WouterDeCoster]

Treated and untreated also is a trait. Your traitfile could very simply contain a 1 for treated and a 0 for untreated.

[Nitin]

Thanks for reply. I gave a thought about it now you gave me confidence to go ahead in that direction :)

[informatics bot]

You don't even need a trait file. You could just run a simple correlation between the module eigen-values (moduleEigengenes()) and the 1 for treated and 0 for untreated "phenotype vector" (Just make sure the samples are in the same order).

Answer 1

[informatics bot]

Take the genes in each module and query them using a gene ontology tool such as: DAVID, GOSTAT, REACTOME, ENRICHR or PANTHER. This will give you clues and information about what each module represents biologically, as well as p-values to measure significance.

[Nitin]

Thanks for reply, I already did this analysis using CLUEGO. I was just thinking if there is any other way to determine module significance using WGCNA.

[informatics bot]

Significance for what? What is your hypothesis?