Illumina cpg probe ID conversion to chromosomal location?
1
2
Entering edit mode
8.6 years ago

from where to get mouse 450K cpgmethylation annotation file Please guide me if any one having information related to this and what are gold standards for annotatiopn

I want to get list of all the CpG probes of Illumina 27K or 450K whatever is available.Then I want to get the chromosome number, genomic position that each probe represents.. I am doing for mouse genome . Can any one help me how to do it? Can I use some data base to download CpG probe list of mouse or some package?

ChIP-Seq • 13k views
ADD COMMENT
1
Entering edit mode

I'm not sure if they have mouse data, but for human, you can download from this link below.

http://support.illumina.com/array/array_kits/infinium_humanmethylation450_beadchip_kit.html

Click 'Infinium HumanMethylation450K v1.2 Product Files', then you will see the manifest file in csv format.

ADD REPLY
0
Entering edit mode

nice. this manifest contains CHR, COORD, SNP, and even some gene symbol annotations

ADD REPLY
0
Entering edit mode

thanks but there I am not finding manifest file in .csv format. there is only .opa format of manifest file and that is for mouse snp. could u plz be kind enough to check that

ADD REPLY
0
Entering edit mode

from where to get mouse 450K cpgmethylation annotation file Please guide me if any one having information related to this and what are gold standards for annotatiopn

ADD REPLY
1
Entering edit mode

I don't think they do a mouse array. This Illumina page http://support.illumina.com/downloads/infinium_humanmethylation450_product_files.html has manifest files (in csv and bpm format) for the human array, and an excel file with the explanation of the column headers for the manifest file.

ADD REPLY
0
Entering edit mode

Dear Mastal511, do you know how we can download these files? They are not available for download. "This system is for the use of authorized users only. " Thank you.

ADD REPLY
0
Entering edit mode

so,from where can i get such type of info for mouse methyalted cpg.Can I use the cpgislands list from UCSC as probes for this purpose and can calculate such information by using some functions available in R package.

ADD REPLY
0
Entering edit mode

I dont know wether they have any mouse array or not? Does any one know?

ADD REPLY
0
Entering edit mode

Google says no. Did you not check before posting?

ADD REPLY
0
Entering edit mode
8.6 years ago

so,from where can i get such type of info for mouse methyalted cpg.Can I use the cpgislands list from UCSC as probes for this purpose and can calculate such information by using some functions available in R package.

ADD COMMENT
0
Entering edit mode

Does Illumina have a mouse array?

ADD REPLY

Login before adding your answer.

Traffic: 1879 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6