Entering edit mode
8.6 years ago
linhuixinnn
•
0
hi,
i am trying to align locally the de novo assembled contigs against the nr database using the blastn program in blast+ suite for annotation purpose. however i received this error that i cant find a solution:
$ /Volumes/1TB\ HDD/Transcriptomes\ Work/ncbi-blast-2.2.26+/bin/blastn -query WT.fa -db chlorophyta -out WT_chlorophyta -evalue 0.0001 -outfmt 6
Critical: (110.6) CNcbiRegistry: Syntax error in system-wide configuration file: NCBI C++ Exception:
"/am/ncbiapdata/release/blast/src/2.2.26/IntelMAC-universal/c++/GCC401-ReleaseMT--IntelMAC-universal/../src/corelib/ncbireg.cpp", line 605: Error: ncbi::IRWRegistry::x_Read() - Invalid registry entry format: '“[BLAST]”' (m_Pos = 1)
Error: NCBI C++ Exception:
"/am/ncbiapdata/release/blast/src/2.2.26/IntelMAC-universal/c++/GCC401-ReleaseMT--IntelMAC-universal/../src/corelib/ncbireg.cpp", line 605: Error: ncbi::IRWRegistry::x_Read() - Invalid registry entry format: '“[BLAST]”' (m_Pos = 1)
please kindly advise. thank you.