Using COG database

Question

[glady]

Hello everyone, I want to assign COG categories to protein of my bacterial genomes. I have already done completed annotation part of the genome. But I'm finding some difficulties to work with COG, since there is proper way out of using COG. If someone of you have worked with COG before, please help me with this.

Answer 1

[bpgatool]

WebMGA can do that for you.
You just have to plot it as you wish.

OR
get cog database from here and align it with your sequences by local blast program and find best hits based on identity, bit score or coverage yourself. Hits will have information about the cog id which can be linked to COG category for better display.

[glady]

WebMGA takes weeks to process COG on your samples. Is there any way by which we ourself can use the COG ?

[bpgatool]

I edited the answer.

[pbigbig]

Hi, If I already have COGs assignments (in form of list) and I just want to categorize them into each of 25 categories, which tools could help that?

[hizbullahmarwat95]

Hi,how can use COG database for essentiality fuhrer protein analysis,for checking tru homologus, with COG database bacteria.