TF and Promoter

Question

[Azhar]

I have a novel TF binding site related to my study to important gene promoter so i want to confirm that but i don,t have those samples so what kind of molecular techniques i can use?

Answer 1

[Shicheng Guo]

Download TF and TFBS ChIP-seq data from UCSC:

http://hgdownload.cse.ucsc.edu/goldenPath/hg19/encodeDCC/wgEncodeRegTfbsClustered/

And then try to find the cell type you want to validate and therefore you can get what you want to validate.

[Azhar]

I have chip seq data for a kind of human tissue , what I already visualized my peak file from ucsc encode data so i ask what should i do experimentally to vaildate

[Shicheng Guo]

clone the peak region into a report system (vector) and check the fluorescence signal.