getting GQ values with VCF using SAMTOOLS Mpileup
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8.5 years ago
always_learning ★ 1.1k

I am using SAMTools Version: 1.3 (using htslib 1.3) , How to get GQ score for my variants ? I have used this command. samtools mpileup -DguS -f PMC01-01.bam | bcftools call -vmO z -o PMC01-01.vcf.gz

SAMTOOLS • 2.8k views
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8.5 years ago

Add -f GQ to the bcftools call command.

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How to set values for Filter columns with SAMTOOLS vcf files. I am getting "." for all VCF entries.

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