Is there a source where I can get directly usable GTF file HG19 for RNA seq which is compatible with Cufflink.
Is there a source where I can get directly usable GTF file HG19 for RNA seq which is compatible with Cufflink.
Here you can find : http://genome.ucsc.edu/cgi-bin/hgTables?command=start
GENCODE annotations should work fine with Cufflinks:
Note: Look for a version of annotations for hg19.
You can also reformat another GTF to work with Cufflinks. Cufflinks requires the 9th field to be of minimum format:
gene_id "Gene_ID"; transcript_id "Transcript_ID";
There is a release for GRCh37 (hg19): http://www.gencodegenes.org/releases/24lift37.html
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You could get it out of iGenomes bundle.
. Or from TableBrowser (from UCSC) or from Ensembl here.