gtf file for cufflink
2
0
Entering edit mode
8.5 years ago
rob.costa1234 ▴ 310

Is there a source where I can get directly usable GTF file HG19 for RNA seq which is compatible with Cufflink.

RNA-Seq • 3.2k views
ADD COMMENT
2
Entering edit mode

You could get it out of iGenomes bundle.
. Or from TableBrowser (from UCSC) or from Ensembl here.

ADD REPLY
0
Entering edit mode
8.5 years ago
BioRyder ▴ 220

Here you can find : http://genome.ucsc.edu/cgi-bin/hgTables?command=start

ADD COMMENT
0
Entering edit mode
8.5 years ago
robm9119 ▴ 180

GENCODE annotations should work fine with Cufflinks:

http://www.gencodegenes.org/

Note: Look for a version of annotations for hg19.

You can also reformat another GTF to work with Cufflinks. Cufflinks requires the 9th field to be of minimum format:

gene_id "Gene_ID"; transcript_id "Transcript_ID";

ADD COMMENT
0
Entering edit mode

There is no version for hg19.

ADD REPLY
0
Entering edit mode

There is a release for GRCh37 (hg19): http://www.gencodegenes.org/releases/24lift37.html

ADD REPLY
0
Entering edit mode

Yes, but those don't have "chr", so most tools will not recognize if the BAM is real hg19.

ADD REPLY

Login before adding your answer.

Traffic: 2880 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6