gtf file for cufflink
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8.5 years ago
rob.costa1234 ▴ 310

Is there a source where I can get directly usable GTF file HG19 for RNA seq which is compatible with Cufflink.

RNA-Seq • 3.2k views
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You could get it out of iGenomes bundle.
. Or from TableBrowser (from UCSC) or from Ensembl here.

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8.5 years ago
BioRyder ▴ 220

Here you can find : http://genome.ucsc.edu/cgi-bin/hgTables?command=start

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8.5 years ago
robm9119 ▴ 180

GENCODE annotations should work fine with Cufflinks:

http://www.gencodegenes.org/

Note: Look for a version of annotations for hg19.

You can also reformat another GTF to work with Cufflinks. Cufflinks requires the 9th field to be of minimum format:

gene_id "Gene_ID"; transcript_id "Transcript_ID";

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There is no version for hg19.

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There is a release for GRCh37 (hg19): http://www.gencodegenes.org/releases/24lift37.html

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Yes, but those don't have "chr", so most tools will not recognize if the BAM is real hg19.

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