Experimentally proven of genes related to one GO term

Question

[Omid]

How can get a gene list(which is reliable and experimentally proven) for a GO term in human?

Answer 1

[Jean-Karim Heriche]

GO annotations of genes are associated with evidence codes so you could filter your gene list for genes that have annotations with an experimental evidence code that suits you. Alternatively, you could use UniProt, they also use GO terms and experimental evidence codes but also keep track of the source paper (via its PubMed ID) which GO is actually also doing.

[Omid]

Could you please how exactly I can filter based on experimental evidence code? I found GO terms but I want to filter based on evidence codes and also in human/mouse. Thanks

[Jean-Karim Heriche]

Download the gene annotation file for your species of interest from the GO download page. The evidence code is in one of the columns.