tools for gene assembly and quality check for sanger sequencing

Question

[lait]

Hi,

Can anyone advise me please, what is the best tool to use to perform gene assembly after performing sanger sequencing? we aim to sequence around 7 genes using sanger sequencing.

and one more question, is there another tool to check the quality of the fragments before assembly ? (like the case of fastqc in NGS).

many thanks

Answer 1

[ismaeilmohamed304]

http://www.dnabaser.com/

[lait]

do you know about any free tools ?

Answer 2

[Benn]

Did you try Phred and Phrap?

http://www.phrap.org/phredphrapconsed.html

Answer 3

[ismaeilmohamed304]

use dna basser it's for free up to 30 days