Microarray analysis--exprs has probe IDs, but the column has no "ID" designation.
0
0
Entering edit mode
8.5 years ago

Hello,

I am doing a microarray analysis. I analyze my .CEL files, normalize them with gcrma, but after I convert the data to an expression set, the probes are listed but the column has no name.

# load files
celfiles <- read.affy(covdesc="phenodata.txt", path="data")
celfiles.gcrma <- gcrma(celfiles)

eset <- exprs(celfiles.gcrma)
eset
             GSM475246.CEL GSM019472.CEL GSM583543.CEL GSM885321.CEL GSM678897.CEL
1415670_at        8.996238      9.145484      9.042686      9.256164      9.319093
1415671_at        9.908269      9.780557      9.861036     10.114690     10.078675
1415672_at       10.435323     10.369950     10.491089     10.381307     10.524440
1415673_at        6.744400      6.698366      6.543279      6.711245      6.634974
1415674_a_at      8.027900      8.121387      7.981725      7.948881      8.070554

From there, after finding differentially expressed genes, the probe column still has no value I can call with $.

                 logFC   AveExpr         t      P.Value    adj.P.Val         B
1449015_at   -3.674883 12.439952 -46.19625 5.123217e-10 6.569675e-06 10.996695
1450276_a_at -4.343238  4.496829 -45.34939 5.835043e-10 6.569675e-06 10.953795
1418165_at   -3.909790  7.624427 -38.77655 1.753611e-09 1.316260e-05 10.543996
1416456_a_at -3.256041 10.883295 -35.44438 3.295267e-09 1.855070e-05 10.268945
1422876_at   -2.739863  3.623318 -33.10276 5.322602e-09 2.128102e-05 10.039264
1418207_at   -3.440342  4.708131 -32.80529 5.670403e-09 2.128102e-05 10.007586
1430899_at   -4.513655  5.071580 -31.48338 7.565404e-09 2.433682e-05  9.859246
1419725_at   -5.585713  7.749529 -30.63524 9.160899e-09 2.578564e-05  9.757116
1459889_at   -4.106095  4.454395 -29.81515 1.107854e-08 2.771851e-05  9.652759
1425451_s_at -3.646441 11.884753 -28.92088 1.371148e-08 3.087550e-05  9.532222

Massive thanks in advance for anyone that can help with this.

microarray bioconductor affy limma • 1.8k views
ADD COMMENT
2
Entering edit mode

You can use rownames to extract probe column.

ADD REPLY
0
Entering edit mode

Thank you so much, it worked. I love this community so much, you never fail me.

Thank you, thank you, thank you. :)

ADD REPLY

Login before adding your answer.

Traffic: 2683 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6