Entering edit mode
8.5 years ago
kelvinfrog75
▴
10
Hey, I got a question about using blast program for assembled contigs (in fasta format) of metagenomics samples. I saw many metagenomics papers from other groups used blastx to blast the contigs against reference protein database (which is virulence gene of bacteria). I just wonder what is the advantage of blastx over using blastn to blast the contigs against reference nucleotide? In my case, I saw there is a big difference between the results from blastx and blastn. I think my sample has a lot more hit when using blastx, so why blastx give me more hit than blastn?
