Strand Specificity and Identification of Antisense RNA

Question

[vimlakany]

HI I have a set of SRA data (Paired end), for which is it possible to find strand specificity using tools or softwares? And how to identify Antisense RNA and trans-encoded RNA?

Answer 1

[Devon Ryan]

You can use RSeQC to estimate whether the library is stranded or not. Antisense RNA is anything on the antisense side, so write a program for it (they probably already exist, but this is a very simple thing to write).