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8.5 years ago
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Hello,
Im running CummRbund on R but having a weird issue when generating heatmaps. I load in my files, all the functions seem to be working fine (dispersion plot, volcano plot etc), but when I input a list of genes using the following command:
myGenesId <- c("NM_021279","NM_001256224", "NM_023653","NM_009520","NM_009521", "NM_009522")
myGenes<-getGenes(cuff,myGenesId)
h<-csHeatmap(myGenes)
h
CummRbund generates a heatmap but some/most of the genes listed are missing. I've gone back to the original tabular file to check if all of those genes are there and they are with quite high FPKM values so not sure why it just omits some of them.
Any help would be really appreciated. Thanks
Did you check if you have all the genes in the myGenes object? In case, can you upload your dataset?
Yes, looking at the myGenes object lists all 18 of the variables i've added but the heatmap only generates 5 of them.