Hi all friends,
I have a dumb question, sorry for it. I focus on a gene with multiple transcripts from human, I want to know the position (start and stop points) of each transcript in the genomic sequence of the gene. Could you please help me what I can do?
Thank you
I assume you have a sam file of the alignments? Here is the pdf for the format: https://samtools.github.io/hts-specs/SAMv1.pdf
Column 4 holds position information of the aligned read, and length of the read can be obtained from column 10. This should be enough information to filter all reads from a given position on a genome.
Hope that helps!
This is not providing an answer for the question asked in the original post. I have moved this post to a comment.