Entering edit mode
8.5 years ago
cristina_sabiers
▴
110
well after medhat told me my vcf doesn't have header I try to figure out how to get it, my vcf file I got it with two ways
bin/freebayes --fasta-reference /media/cri/CRIS_DATA/ULT/OTRO2/otro/hg19.fa /media/cri/CRIS_DATA/ULT/OTRO2/otro/input.header.realigned.bam >/media/cri/CRIS_DATA/ULT/OTRO2/otro/freebayessample3.vcf
bin/freebayes --min-coverage 10 -V -f /media/cri/CRIS_DATA/ULT/OTRO2/otro/hg19.fa /media/cri/CRIS_DATA/ULT/OTRO2/otro/input.header.realigned.bam >/media/cri/CRIS_DATA/ULT/OTRO2/otro/freebayessample.vcf
Is any command to add to get my head on the file?
Thanks
FreeBayes outputs VCF headers by default (at least, the current version does). Are you sure the VCF with missing header is direct output from FreeBayes, or after additional processing (e.g., BEDtools 'subtract' removes headers)?
from the documentation page the command is
and it should have the header by default what was the command you used for the alignment and is it was to the same reference