How to estimate isoform expression of a certain cell type given multiple samples?

Question

[Vivian]

I know this question may sound naive, but I'm not a biologist so I would like to know if my idea sounds reasonable to the biology community.

If I have multiple RNA-seq samples from the same cell type, does it make sense to say that I want to estimate the isoform expression levels in this cell type? All the current methods I know perform estimation on each sample separately. What if I want a final answer for the cell type, not a set of values for each sample? Can I assume samples from the same cell type have the same isoform expression, ignoring the sequencing biases?

Thanks in advance!

Answer 1

[EagleEye]

Check this post

A: workflow/tool for alternative splicing