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8.3 years ago
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I was running this tool called ContEst ( https://www.broadinstitute.org/cancer/cga/contest_run ) by Broad Institute to determine NGS sample contamination. I ran the analysis twice and got two different results for the same samples. The contamination level I am getting for the first analysis is less than 0.5% and in the second analysis, it's more than 97% for the both samples. Can someone please explain the reason for these variegated results?
First Analysis results:
AOGC_NAME population population_fit contamination confidence_interval_95_width confidence_interval_95_low confidence_interval_95_high sites
S09-F09-P01 CEU n/a 0.3 0.1 0.3 0.4 4498
AOGC-02-0112 CEU n/a 0.5 0.1 0.5 0.6 4870
Second Analysis results:
AOGC_NAME population population_fit contamination confidence_interval_95_width confidence_interval_95_low confidence_interval_95_high sites
S09-F09-P01 META CEU n/a 97.8 0.4 97.6 98 4208
AOGC-02-0112 META CEU n/a 97.7 0.6 97.4 98 4619