How sense strand is deciphered after sequencing?

Question

[utkarsh.sood]

Hello,

I have a query that, how sense strand is deciphered after we get the sequence of a bacterial genome sequenced by SMRT?

Thanks!

[piet]

Bacterial replicons like chromosomes or plasmids encode many genes and the orientation of these genes is not much restricted. Neighboring genes often have opposite orientation. Therefore the concept of a "sense strand" is not useful when talking about complete bacterial chromosomes or plasmids.

Answer 1

[WouterDeCoster]

I'm not aware of a protocol for strand specific genome sequencing. Furthermore, does that even make sense? Corresponding to (m)RNA there is a sense and antisense strand related to from which strand the RNA is transcribed, but is this something you can say about an entire bacterial genome?