Hi everyone, I have a question and I want to learn follow a what kind of a way
I have a gene, for example ACTN3 I want to take ACTN3 gene expression level, in maybe stress, maybe exercise, maybe stable time but different tissue
Other need, I want to multiple sequence alignment ACTN3 and its whole variant for example its snp that cause stop codon, I search in NCBI I found more than 10 but I couldn’t alignment them, because I count get their full fasta format, what should I do?
Finally, I alignment a gene and I wanted to compare with other organism, I got some differences but when I got 3d protein model, Swiss model give me only human protein, other protein exactly like it and I detected Swiss model show what it have whenever i search, i didn’t modeling based on my protein seq data, isn’t it
Thank you all answer already now, and sory for my english
That's a lot of questions, and I get the general expression that your background in bioinformatics is rather limited. I would suggest to start with just one of the things you stated above. Can you elaborate on which data you have and what you aim to achieve?
actually I just need doıng multıple seq alignment a gene and with its variants and SNP, after select important variance and finally say something before experiment looking result, for example a gene which is coding an enzyme and its variant coming with stop codon and protein coding very complicated, I can say, a binding site is closed so enzyme is less active then original form
So this no longer has anything to do with expression levels?
You need to do multiple sequence alignment of what? And what is the aim of your project? It's all rather unclear to me. What is your background?
It was different question but I give up :D, Look I have a gene, and I have a lot of polymorphism that is unknown what it causes, I want to suppose looking for differences between orjinal gene or protein to polymorphisms