Outcome of adding a PolyUU tail, in reads that have to be sequenced, in the sequenced reads
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8.3 years ago
fusion.slope ▴ 250

Dears,

i have a question about DNA sequencing:

if i add a poly U tail of 2 nucleotides in my reads at the 3' end and these reads have to be sequenced, what i would expect in the "sequenced" read?

Two TT or Two AA at the '5 start ?

Thanks in advance for any reply

sequencing • 1.2k views
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8.3 years ago
fusion.slope ▴ 250

i got the answer from colleagues: two AA at '5 start..

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