CPC Error Can't find blastall on your path, eheck it!

Question

[sdf.ambreen]

When i run cpc cd $CPC_HOME time cpc-0.9/bin/run_predict.sh Trinity.fasta test.out $HOME/testdir test.ev

Error

Can't find blastall on your path, eheck it! Can't find framefinder on your path or my own directory, quitting...

kindly tell me how to solve it

[Medhat]

This is a question not a tool, right?

Answer 1

[sxzhuxu]

Hi,

I encountered the same error when running CPC.

Because you use Blast+ package which do not have formatdb and blastall. You should download a legacy version.

I find the legacy blast package in ftp://ftp.ncbi.nlm.nih.gov/blast/executables/legacy/2.2.9/

wget -c ftp://ftp.ncbi.nlm.nih.gov/blast/executables/legacy/2.2.9/blast-2.2.9-amd64-linux.tar.gz
mkdir blast && tar -xvzf blast-2.2.9-amd64-linux.tar.gz -C ./blast
cd blast
ls
bl2seq    blastclust      blastpgp   copymat  fastacmd      formatdb      impala      makemat    megablast.txt  rpsblast      seedtop
blastall  blastclust.txt  blast.txt  data     fastacmd.txt  formatdb.txt  impala.txt  megablast  README-qm      rpsblast.txt  VERSION
echo "export PATH=`pwd`:\$PATH" >> ~/.bashrc && source ~/.bashrc

Here, you can find the expected script blastall. You can add this path to PATH.

I hope this would be useful for you.

Zhongxu