Entering edit mode
8.3 years ago
Edalat
▴
30
HI, I am analyzing miRNA (small RNA analysis) data and I would like to establish a fold-change and p-value threshold which is biologically significant.with Fold change ≥ 1.5 and FDR P-VALUE ≤ .05, I lose most of miRNA.
thank you for attention. with FDR P-VALUE ≤ .05 of t-test (without fold change) , I lose most of miRNA too, I think to use bigger p value?
Then I will see how many replicates you have. It looks like you have few replicates and which do not show a good correlation with in a group
excuse me which is better for filtering miRNA ,FDR P-value or P-value?
I read many things about being cautious with p-value then I prefer FRD