Entering edit mode
8.3 years ago
omidrouhi
•
0
Is it possible to automate the generation of a vcf for:
chr7 140453136 . AC TT 100.00 PASS
instead of:
chr7 140453136 . A T 100.00 PASS
chr7 140453137 . C T 100.00 PASS
Regards,
OR
you must give the context of your query ( used tools, organism,... ); how do you know that both mutations belong to the same variant ?
It is the human,a formalin fixed paraffin embedded tissue. It would require the variant calling tool that generates the vcf to recognize that the AC>TT is on the same stand (i.e. cis). If the A>T and C>T are on different strands (i.e. trans), AC>TT would be a miscall. What tool can I use?