is there a DB for illumina adaptors/contamination?
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8.3 years ago
blur ▴ 280

Hi,

trying to figure out of this repetitive seq in my data is a contamination or not: tggggaaatcgcaggggtcagcacatc-cggagtgcaatggataagcctcg any ideas where I can find a DB? (It is not automatically recognized as an illumina adaptor)

Thanks

sequencing RNA-Seq • 1.6k views
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8.3 years ago
SJ Basu ▴ 60

you can find find all possible adapters and contaminants from Illumina in the "Configuration" folder of FastQC tool (also create a reverse complement of all those adapter sequences and check )

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8.3 years ago
Benn 8.4k

What about NCBI blast db?

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8.3 years ago
dago ★ 2.8k

Maybe previous posts Finding Adapters For Illumina Reads and blogs help

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8.3 years ago

According to blast, both are snRNA/ncRNA

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