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8.2 years ago
saranpons3
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70
Dear Friends, I am a beginner in the field of De novo Genome Assembly. I tried to understand coverage cut off and expected coverage parameters in Velvet assembler. But i could not understand!. Could anybody explain me what is coverage cutoff and expected coverage in denovo assembly with an example? Thanks in advance.
In my humble opinion velvet is extraordinarily difficult to use and I would recommend to start learning genome assembly with a more user friendly program like spades. Assemble some coli genomes and then play around with different kmer sizes and see how it effects the result.
Hi, Thanks for your reply. I will consider your advise and try to use Spades
this might help
Hi, Thanks for your reply. i had read what is given in the link. But i could not understand. Could you give me some examples if you have any to understand Coverage cutoff and expected coverage.?