miRNA DE analysis with mirdeep2

Question

[zizigolu]

hi,

I want to provide read counts file for edger analysis. then by this syntax from mirdeep2

quantifier.pl -p precursors.fa -m mature.fa -r reads.fa -s star.fa -y now -t cel

but in my organism " Aspergillus fumigatus" how I can find precursors.fa and mature.fa files????

in mirbase there is not my desire organism :( :( :(

Answer 1

[harold.smith.tarheel]

Obviously, you have to create the precursor and mature files yourself, perhaps based on synteny/sequence similarity plus transcript data.

[zizigolu]

thank you, you mean I should blast what on what please?