Blast Fatal Error -Database Not Found

Question

[RAKESH SARKAR]

I am using blastall 2.2.26 on ubuntu for local databse blast for

Query: Osci-ncku.cat.aa.fa;

Database: Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa;

With my database files in the same directory, but still I am getting FATAL ERROR

$ ls Osci-ncku.cat.aa.fa Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa.phr Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa.psq Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa.pin formatdb.log

$ blastall -p blastp -d Osci-ncku.cat.aa.fa -i Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa -F F -m 8 -o Ref1_Osci.out -b 1 -v 1

[blastall] FATAL ERROR: AFY79762.1: Database Osci-ncku.cat.aa.fa was not found or does not exist

Am I grossly missing out something. Any suggestions on how to trouble shoot this. Thank you.

Answer 1

[Prasad]

Your input file and database files are exchanged. -d should be Oscillatoria_acuminata_pcc_6304.ASM31710v1.pep.all.fa and -i should be Osci-ncku.cat.aa.fa