BUSCO for transcriptome assessment

Question

[Mehmet]

Dear All;

I used BUSCO (BUSCO_v1.22) to check my transcriptome assembly produced by Trinity. I received the error below. Has any of you tried to use BUSCO or received the same error?

Error: (1431.1) FASTA-Reader: Warning: FASTA-Reader: Title is very long: 1390 characters (max is 1000)/

and the output file of BUSCO:

BUSCO was run in mode: trans

Summarized benchmarks in BUSCO notation: C:0%[D:0%],F:0%,M:0%,n:429

320 Complete BUSCOs
267 Complete and single-copy BUSCOs
53  Complete and duplicated BUSCOs
22  Fragmented BUSCOs
87  Missing BUSCOs
429 Total BUSCO groups searched

[Israel Barrantes]

Did you even google the error message? Because there are solutions given on StackOverflow and here as well.

[Mehmet]

I did, I mean why the percentages are zero.

Summarized benchmarks in BUSCO notation: C:0%[D:0%],F:0%,M:0%,n:429

[Israel Barrantes]

Your question literally was about the error. You did not ask why you were getting zeroes at all.

And the answer should be linked to checking long/short annotation names, as in the error. But anyway, you don't need the numbers there, as they are also printed below, e.g. C should be 320/429 = 0.7459, or barely 75%.