Sanger sequencing data analysis

Question

[adrian.jose09]

Free software which you recommend for Sanger sequencing data analysis.

Best regards

[John]

Hi Adrian - could you be a little more specific? What kind of data analysis? Also i've changed this from Tool to Question as Tool is used for people releasing new tools rather than asking for them :)

[Mehmet]

Also, which platform? illumine, pacbio, oxford etc. Next Generation Sequencing ?

[WouterDeCoster]

Sanger sequencing. Besides the lack of information in the question, this part seems rather clear.

[adrian.jose09]

Is Sanger Sequencing and i need EST Analysis tools

[WouterDeCoster]

Please use ADD COMMENT and ADD REPLY to answer to earlier posts, as such this thread remains logically structured and easy to follow.

[looi_daniel]

Hi, I am planning to use NovoSNP for SNP detection. However, I faced some challenges inserting the refseq. Could you kindly advice me how to perform the run ?

Thank you.

[WouterDeCoster]

I faced some challenges inserting the refseq

Can you be more specific? With this information I don't know what goes wrong. We usually download a .embl file from ensembl for our gene of interest.

Answer 1

[WouterDeCoster]

We use novoSNP (developed in-house as well).

[trausch]

The novoSNP URL appears to be dead. Is there an updated one?

[WouterDeCoster]

I'm sorry for the delay, but I have now changed the link to its updated location.

Answer 2

[trausch]

We recently developed Tracy to analyze Sanger sequencing data, works from the command-line and using web applications.