FASTX error - quality score
0
0
Entering edit mode
8.1 years ago
blur ▴ 280

Hi, I am using the FastX collapser option, with the Q33 option, as my reads were sequenced by illumina and yet I get an error msg: "invalid quality score data..."

the command I ran was fastx_collapser -Q33 -i infile.fq -o outfile.fq

Thanks!

FASTX • 2.3k views
ADD COMMENT
0
Entering edit mode

The part of the error message that you did not quote after the "..." probably contains relevant information. Can you fully quote the message and provide a minimal test example ?

ADD REPLY
0
Entering edit mode

I figured it out - the file was corrupted, and a line existed with only quality scores and no sequence data.... Thanks!

ADD REPLY

Login before adding your answer.

Traffic: 1900 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6