Entering edit mode
8.1 years ago
Biogeek
▴
470
Hi,
I've used RAPsearch2
in -s F
mode, which equates to NCBI blastX e-value output style. I have used a threshold of 0.00001. 1x10-5..some of my blast hits I get are as follows: I'm getting e values which start as -(number)e+08. Usually I get e-values of ( number)e-(number). Stupid question but -3e+08 equals?
# RAPSearch
# Job submitted: Fri Sep 30 11:44:18 2016
# Subject : rap_uniprotcompletedb
# Fields: Query Subject identity aln-len mismatch gap-openings q.start q.end s.start s.end e-value bit-score
_000014t1 sp|Q8NEZ4|KMT2C_HUMAN 57.1 7 3 0 8085 8065 988 994 -3e+08 16.5
_000014t1 sp|Q6RSS2|L_PIRVV 27.6 29 21 0 11256 11342 322 350 -3e+08 16.5
_000014t1 sp|Q9UIF8|BAZ2B_HUMAN 45.5 11 6 0 5450 5482 1796 1806 -3e+08 16.5
_000014t1 sp|Q82081|POLG_HRV3 50.0 8 4 0 5272 5249 1837 1844 -3e+08 16.5
may i add... the sequences with these values are rather large. i.e +10k bp in size.
the sequences might be large the hits (HSPs) reported by rapsearch are rather small (7-29 in length).
So I assume they will have a poor evalue, but a negative one is indeed rather weird (I don't think that's even possible).
-3e+8 corresponds to -300000000 btw