weird e-values with rapsearch
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8.1 years ago
Biogeek ▴ 470

Hi,

I've used RAPsearch2 in -s F mode, which equates to NCBI blastX e-value output style. I have used a threshold of 0.00001. 1x10-5..some of my blast hits I get are as follows: I'm getting e values which start as -(number)e+08. Usually I get e-values of ( number)e-(number). Stupid question but -3e+08 equals?

# RAPSearch
# Job submitted: Fri Sep 30 11:44:18 2016
# Subject : rap_uniprotcompletedb
# Fields: Query Subject identity        aln-len mismatch        gap-openings    q.start q.end   s.start s.end   e-value bit-score
_000014t1 sp|Q8NEZ4|KMT2C_HUMAN   57.1    7       3       0       8085    8065    988     994     -3e+08  16.5
_000014t1 sp|Q6RSS2|L_PIRVV       27.6    29      21      0       11256   11342   322     350     -3e+08  16.5
_000014t1 sp|Q9UIF8|BAZ2B_HUMAN   45.5    11      6       0       5450    5482    1796    1806    -3e+08  16.5
_000014t1 sp|Q82081|POLG_HRV3     50.0    8       4       0       5272    5249    1837    1844    -3e+08  16.5
rapsearch e-values • 1.8k views
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may i add... the sequences with these values are rather large. i.e +10k bp in size.

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the sequences might be large the hits (HSPs) reported by rapsearch are rather small (7-29 in length).

So I assume they will have a poor evalue, but a negative one is indeed rather weird (I don't think that's even possible).

-3e+8 corresponds to -300000000 btw

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