From gene symbol (gene name), TSS id and locus coordinates .. How to get the Refseq RNA (RNA accession number .. NM *******)?!!
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8.1 years ago

Dear All, I got RNAseq data already processed. I have txt file containing "Official gene symbol", "TSS id" and "locus coordinates" and relative "FPKM" value. I need to have the "gene id" (that's fine) and also the Refseq RNA id (NM ....), because I NEED TO KNOW WHICH ISOFORM has this FPKM value. PLEASE help me to understand how can I get this information from gene symbol, TSS id and locus!!

RNA-Seq • 5.6k views
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Always useful to post an example snippet and specify what genome this data is from.

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I apologise! Human Genome, in particular MCF-7 cell lines.

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You may want to speak with the people who processed the data. It is possible that they generated this at the gene level, rather than the transcript level.

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I'm pretty sure not all isoforms can be uniquely identified by the information you describe you have.

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This one http://hgdownload.soe.ucsc.edu/goldenPath/hg19/database/refGene.txt.gz has Chromosome, strand (+ or -), gene_id, gene_symbol and RNA accession number. But I couldn't understand the other columns .. I need the locus coordinates; to link them to the different RNA accession number.

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