Off topic:How to plot "normalized read density" vs "insert size"
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8.4 years ago
dec986 ▴ 380

Hello,

I've been requested how to make a plot Plot like this, which is from an ATAC-Seq methods paper (Buenrostro, J.D., et al. doi: 10.1002/0471142727.mb2129s109) of RNA-Seq Data I'm not sure how I can get the insert size and then the normalized read density. I've tried rseqc's insertion_profile.py but there are bugs in that script so I can't use it.

How can I go about making getting this data from fastq or aligned bam files?

RNA-Seq • 2.0k views
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