Can I assemble a Eukaryote RNA-Seq using my laptop?

Question

[utsafar]

I am interested in working on saffron (Crocus sativus). There is a data set related to saffron in SRA database, SRP052616 which contains 5 subsets. I want to assemble its stigma RNA-Seq data, to further work on. SRA file has 4.6G bases and is 3Gb, and when converted to fastq, it is almost 9Gb. Is it possible to assemble this file using my Core i3, 4G RAM, 500G, HDD? If it is possible, please suggest me some applications. I have both windows 7 and Linux Mint 14.7 on my laptop. If it is not possible, please tell me what can I do?

[russell]

Kallisto will (pseudo) align on a laptop

https://liorpachter.wordpress.com/2015/05/10/near-optimal-rna-seq-quantification-with-kallisto/ https://pachterlab.github.io/kallisto/

[GenoMax]

While correct this is not a solution to the question being asked in the original post.

Answer 1

[Antonio R. Franco]

I don't believe you can do it with such a small computer/s

I see you need to assemble a transcriptome

You can do it using Trinity. The authors of this nice program have set a terrific web service based upon Galaxy that will allow to use Trinity for free for your own assembly. I did it, and it worked very well, and in my case, I used 240Gb of RAM memory. You only need to ask for a login and password. The Galaxy portal is here

https://galaxy.ncgas-trinity.indiana.edu/user/login

[utsafar]

I am using this one: http://galaxy-qld.genome.edu.au/galaxy