VelvetOptimiser getting an unknown option error

Question

[Pryce Michener]

I'm trying to run VelvetOptimiser, and I keep getting the following error:

VelvetOptimiser.pl Version 2.2.5

Number of CPUs available: 32

Current free RAM: 122.174GB

Velvet OMP compiler setting: 1

Unknown option: fasta -shortpaired pairedreads_paired.fasta -fasta -short singlereads_singletons.fasta

This is my command:

perl ./programs/VelvetOptimiser-2.2.5/VelvetOptimiser.pl –s 51 –e 71 –o'-ins_length 350' -f '-fasta -shortPaired PairedReads_paired.fasta -fasta -short SingleReads_singletons.fasta'

Does anyone know what is happening here? For some reason it's getting rid of the "-" in front of fasta in the velveth options, but I can't seem to get it to work at all.

[Sej Modha]

Are you sure you have not copied and pasted the command from web or any other documents, as it sounds like a typical copy-paste command issue. Make sure that you type the entire command on the terminal and check if the error disappears!