Hi all, I downloaded a set of mammalian ageing-related gene expression data sets (GDS files) from GEO using the filter:
(("age"[Subset Variable Type]) OR "development stage" [Subset Variable Type]) AND "mammals" [Organism]
Now, I want to download all of the RNA seq data sets for mammalian ageing-related gene expression data sets.
From what I understand, for RNASeq, I can't download the gene expression data sets (GDS files in GEO), I have to download the raw SRA reads (which I presume are like .CEL files), read them in to some software, and obtain the gene expression values myself?
So in the NCBI SRA database, I tried to use this filter:
(("age"[Subset Variable Type]) OR "development stage" [Subset Variable Type]) AND "mammals" [Organism] AND gds_sra[filter]
But I got no results.
I've also seen some other databases, such as ArrayExpress. What I'm wondering (1) Does NCBI SRA database have the data I am looking for and (2) Why does my command to filter and retrieve the set of mammalian ageing-related data sets not work? I assumed that NCBI would have the most comprehensive collection of data, hence why I am focusing on trying to extract data from there.
Thanks
