bcl2fastq to output different lanes together

Question

[luisantome]

Hi there,

I need some advice regarding bcl2fastq usage. I have rapind run HiSeq data where clusters were generated on board. All lanes are identical, so we have same the samples with the same barcodes in every lane. Is it possible to join lectures during demultiplexing? I mean, is it possible to put together all reads for every sample coming from different lanes?

Thank you in advance.

Best, Luis

Answer 1

[GenoMax]

Assuming you are running bcl2fastq v.2.18 use --no-lane-splitting option when you demultiplex.

[luisantome]

many many thanks genomax2!