question about bedGraphToBigWig

Question

[ch8316f5eyu]

genomeCoverageBed -ibam SRR2048235.bam -bg -trackline -split -g mm10.chrom.sizes > mytrack.bedGraph
bedGraphToBigWig mytrack.bedGraph mm10.chrom.sizes out.bw

Those are the processes I used to generate the bigwig file out.bw. After I pasted the url to UCSC, I got this error message:"http://ch8316f5eyu.220idc.cn/test/out.bw is not a bpt b-plus tree index file". Does anyone have the idea what is going on?

Answer 1

[Devon Ryan]

The file became corrupt either during creation or (more likely) during upload. For example there's an extra byte that's inserted at position 6 (value 13) that's shifting the remaining bytes by 1. Reupload the file and see if that solves the problem (if not, delete it and make it again).

[ch8316f5eyu]

Actually I simplify this problem. At first I wanted use Homer to generate multiwig to overlay tracks in UCSC, and I got this problem after paste url in track hub. In a word, I have tried so many times and this problem always occurs............

[Devon Ryan]

Your response serves no purpose. I have already told you (1) why this is happening and (2) possible solutions. The fact that you used Homer is completely irrelevant.

[ch8316f5eyu]

Thanks. You are right. The file worked fine for IGV before uploading, but became damaged when I download this file from this url and did not work for IGV. It is weird that uploading can damage file every time........