Entering edit mode
8.0 years ago
burcakotlu
▴
40
Dear all,
Is there a way to get DNA sequences for a specific assembly (for a given chromosome, start and end coordinates) using ncbi eutils?
String eFetchString = "https://www.ncbi.nlm.nih.gov/entrez/eutils/efetch.fcgi?db=nucleotide&id=" + refSeqId + "&strand=1" + "&seq_start=" + oneBasedStart + "&seq_stop=" + oneBasedEnd + "&rettype=fasta&retmode=text";
My code above get DNA sequences in GRCh38.p6, I guess. (latest assembly right now.) Is there a way to specify the assembly and get DNA sequences in GRCh37.p13 or hg19 using ncbi eutils?
By the way, right now I'm focused on ncbi based solutions since I have coded in that way up to now. But I definitely would like to hear about other ways of doing this for my further studies.
Thanks in advance, Burçak Otlu