Functional annotation of unigenes

Question

[Mehmet]

Dear All:

I have a set of unigenes (~30.000). I want to use blastx for searching the unigenes against to non redundant, swissprot, and COG (cluster of ortho groups). Then, I want to use results in blast2go program for annotation. How can I search against to the databases?

Thank you.

Answer 1

[seta]

You can download the database of interest and NCBI-blast program and conduct locally blastx on the server.

Answer 2

[apa@stowers]

Web interface for NCBI blast

Web interface for NCBI CDD blast (for COGs)

Blast2Go download page