Hi, I'm working with two mouse olfactory organ transcriptome datasets of the same mouse species, but sequenced and assembled using different methods. I wanted to use MUMmer to perform an alignment of the transcriptomes to start to assess the different methods. I tried using NUCmer (which seemed the most appropriate option), but quits at the last step "FINISHING DATA." There's not much in the error message or in the manual that I've found so far to address the problem. If anyone has encountered similar issues in MUMmer I'd love some advice. The error message looks like (below):
4: FINISHING DATA postnuc: postnuc.cc:1417: long int revC(long int, long int): Assertion `Len - Coord + 1 > 0' failed. sh: line 1: 26056 Aborted (core dumped) /programs/MUMmer3.23/aux_bin/postnuc -b 200
ERROR: postnuc returned non-zero
