I need the sequences of 3'UTRs of yeast genes. To my surprise Biomart does not have the information and spits out a "Sequence unavailable" statement for all the genes. In the SGD database,their repository has termed the utr_fasta files as obsolete (http://downloads.yeastgenome.org/sequence/S288C_reference/orf_dna/orf_dna.README) Has anyone else faced this issue - does any one have an updated and recently annotated sequence file for 3'utrs in yeast. Kindly let me know.
While it does say "Sequence unavailable" for majority of entries it is not the case for all genes. You may want to report this to Ensembl's helpdesk.
If you choose exon sewuences instead of 3' UTR, it works fine. Are you sure the UTRs have been annotated in S. cerevisiae?