Off topic:5 column MACS .bed file into a BigBed
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7.2 years ago
varsha619 ▴ 90

Hi, I am trying to convert my 5 column .bed file into a BigBed for loading to UCSC using -

bedToBigBed -type=bed3+2 -tab in.bed chrom.sizes out.bb

My bed file is generated using MACS peak caller so it is of the format - chr start stop MACS_ID MACS_score

The error I am getting is -
pass1 - making usageList (6 chroms): 1 millis
pass2 - checking and writing primary data (127 records, 5 fields): 2 millis
index write: 0 millis
pass3 - writeReducedOnceReturnReducedTwice: 0 millis
further reductions: 1 millis

Has anyone else faced the same issue? Please let me know, thank you.

ChIP-Seq bed ucsc • 1.3k views
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