Why did not BLAST show the results that fit the my requirements?

Question

[2351688559]

Dear all. Recently, I did a BLAST search with e value of 1e-10 as the threshold. Then,by doing the same BLAST search without setting the threshold, I found some hits, of which the e value was below the threshold (1.6e-16), did not show up in the orginal search. Could you please tell me what happened? feel really confused...

[Michael]

Please provide a reproducible example, blast version, input data, parameters.

[Jean-Karim Heriche]

How did you do the searches ? What were the parameters you used in both cases ?

[2351688559]

I wanted to search homologous of cryptochrome from Neurospora crassa in human's genome. Thus, I used the protein sequence of Neurospora's cryptochrome as the query, and proteins collection of human genome as the databse. All of the parameters were default, except the e value in the first search, which was set to 1e-10.