Diamond annotation-setting antisense strands to sense
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7.8 years ago
hlwalsh • 0

Hello. I have successfully used the program Diamond to annotate my de novo transcriptome, however, I am unsure how to identify if the transcripts are in the sense or antisense direction and how to then set them to sense. I was wondering what programs or processes people use to do this after annotation. I had originally used Blast2Go, but I when I open my Diamond annotations in B2G (as an xml format) I cannot see the full protein description and there is a disconnect because when I use the option "Set to Sense" it will not work.

Thanks!

RNA-Seq next-gen blast • 1.3k views
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