How can I experimentally validate LOH?

Question

[deyugb2012]

Using the SNP array data (1.5MCitrus array) we identified some putative LOH(loss of Heterozygosity) regions in some samples.To infer LOH status of each locus of each sample we used genotype calls at that locus compared with a paired sample from a putative progenitor accession. We are expecting that these LOH regions are due to heterozygous deletions. But to confirm it we need to validate this by experiment ( e.g, PCR-based). These LOH regions are ranging from 15-100kb. Can anybody give me some idea how to validate validate these LOH regions?

Thanks

[Brian Bushnell]

Perhaps you can look at the raw probe intensities and see these regions are, on average, weaker? The easiest way would be WGS, but that would be expensive; if you can nail down the start and stop very precisely, you could Sanger-sequence the endpoints.

[vmicrobio]

On what organism are you working on? if it's a microorganism spore dissection (creation of haploid strains) may be a possibility