Recommendations for Full Genome Sequencing Services

Question

[sean]

Hello everyone, Stumbled across this community and looks like a good source of bioinformatic knowledge. As background i studied genetics to PhD level about 20 years ago and have been interested in getting my full genome sequenced for some time. Now that prices are getting affordable looking for a reliable service that doesnt compromise my privacy too much.

Any recommendations for services that any of you have experience with? Pros and cons of the various options?

I see a site called suregenomics but hard to see if they are actually live or not and the founders are software people not much science background. Not necessarily a negative, but makes me pause.

Any guidance much appreciated.

[sean]

Illumina product looks good. Will read more. Thank you for the useful pointer.

[sean]

Any thoughts on services available in SE Asia?

[GenoMax]

This version of Understand Your Genome is in Tokyo, Japan in June. You need to get your sample in by March 24th.

You could also look at BGI, if you need something in SE asia.

[Petr Ponomarenko]

Most big players are readily available with their local labs in Asia: BJI, Novogene, Macrogen, WuXi, you name it.

[sean]

Great info. Will pursue.

Answer 1

[Petr Ponomarenko]

Hi Sean. If you can locate a doctor or a lab to do DNA extraction, then you can send your sample directly to a sequencing lab like Novogene (we use it), Macrogen, etc for sequencing and if you want most of such labs can do alignment and variant calling, some can do variant annotation as well. These are big labs and their prices usually are competitive with very consistent quality.

[Petr Ponomarenko]

Or you can buy Minion from NanoPore and sequence yourself. While less precise and more expensive and requires some lab work and friends with equipment like centrifuges, I think this is a lot of fun.

[sean]

Thanks useful information. Will explore.

[WouterDeCoster]

Current 'rapid' kits require only a pipette and 5-10 minutes for library prep starting from extracted DNA.

[Petr Ponomarenko]

Do you mean there are already kits that can extract DNA from blood or other tissue sample and no special equipment other than a pipette? Wow. This is cool

[WouterDeCoster]

Ah, right, DNA extraction. My sleeping brain started the library prep from extracted DNA, for which my previous statements is true (edited for clarification). Although not yet out, AFAIK they are working on tools to solve the extraction part, too.

[Petr Ponomarenko]

=) I wish you were right

[WouterDeCoster]

The thing they're working on is called "zumbador", in case you're interested. Googling for "zumbador nanopore" gives a few hits, but not a lot of information.

[Petr Ponomarenko]

I know about that concept and SmidgION. But from my understanding, "zumbador" is going to be very much tied with DNA prep to the end, so you will have to use Nanopore. And I am not sure this is the best (at least not what I want), cause having DNA extraction in cheap and miniature form factor enables way more different things

[WouterDeCoster]

Yes indeed, it would be directly connected to the MinION (or SmidgION) flowcell.

Answer 2

[GenoMax]

You may be interested in Understand Your Genome service from Illumina.

If you are fine with donating your genome data publicly then take a look at the Personal Genome Project.